Presenting Authors: Inmaculada Varo’
Authors: Inmaculada Varó , Ignacio Ortea , María Prado – Alvarez , Pablo García – Fernández , Ismael Hachero – Cruzado , Sonia Dios , Camino Gestal 
1. Instituto de Acuicultura Torre de la Sal (IATS), Consejo Superior de Investigaciones Científicas (CSIC)
2. Instituto de Investigación e Innovación Biomédica de Cádiz (INiBICA), Universidad de Cádiz
3. Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones científicas (CSIC)
4. Instituto Español de Oceanografía, Centro Oceanográfico de Vigo
The common octopus (Octopus vulgaris) is an excellent candidate for aquaculture diversification. It is known that temperature has a great effect on early life stages of cephalopods, drivng the development of embryos, yolk absorption rate and growth rate of paralarvae, which increases with the temperature water. In this study, the impact of temperature in cubation on octopus’ embryos EMB (stage XV) and paralarvae after hatching (PL0) were investigated using the proteomic SWATH‐ MS approach to identify proteins or protein profiles as biomarkers to evaluate the welfare in early stages of O. vulgaris. Two temperatures of incubation were compared: high (21 ºC, commonly used in paralarvae aquaculture) and low (16 ºC, as representative of natural conditions). The proteomic analysis quantified a total of 2352 proteins. For EMB, 24 proteins showed significant differential expression between high (H) and low (L) temperature, whereas in PL0 a total of 127 proteins presented significant differences. PCA analysis of all quantified proteins showed a clear separation between development stages (EMB and PL0) and temperature. The up – regulated proteins by high temperature in EMB (11 up) were mainly related with signaling pathways (signal transduction) scavenging and binding activity (metal, ions, tubulin and chitin). Whereas in PL0, the up – regulated proteins (64 up) by high temperature were involved mainly in proteolysis (hydrolysis activity related with protein metabolism), carbohydrate metabolism, nervous system development and neurotransmitter transport, lipid metabolic process, detoxification, extracellular organization and binding (chitin, metals and ATP). These results allow defining characteristic proteomes for EMB and PL0 that are involved in different biological processes associated to temperature.
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