last updated, September 20th 2013

Wednesday, 25th September


11:30 - 12:00 Registration and welcome appetizer

12:00 - 12:10 Welcome (G. Fiorito) and Introduction to the workshop (P. Andrews & G. Fiorito)


Session 1: "Lessons" for cephalopod regeneration studies from other research fields

12:10 - 12:35 A.V. Coelho (Portugal) - Molecular insights into echinoderms regeneration

12:35 - 13:00 I. Kalajzic (USA) - Approaches to understanding mechanisms that regulate bone repair

13:00 - 13:25 P. Netti (Italy) - Novel strategies and processes for engineering biological tissues in vitro and in vivo

13:30 - 14:30 Lunch

14:30 - 14:55 H. Makarenkova (USA) - Dedifferentiation and transdifferentiation: turning back the cellular clock

14:55 - 15:15 P. Imperadore (Italy) - A short overview on the historical contribution of cephalopods to the Regeneration 'Game' in the animal kingdom 

15:15 - 15:30 — Discussion: Identification of key lessons for cephalopod studies 

Session 2: Regenerative abilities of cephalopods and their potential ecological significance

15:30 - 15:50 P. Andrews (UK) - A survey of damage to the arms of Octopus vulgaris taken from the wild

15:50 - 16:10 S. Bush (USA) - The role of arm regeneration in the behavioral ecology of a deep-sea squid

16:10 - 16:30 G. Fiorito (Italy) - The potential behavioural deficits (and their recovery) in cephalopods following injury

16:30 - 16:50 Coffee break

17:00 - 19:00 Session 3: Round table discussion

i. Defining terms: wound healing, regeneration, degeneration , neurogenesis

ii. Which tissues in cephalopods are capable of regeneration, in which species and how good is the evidence?

iii. How should regeneration of an arm or a tentacle be measured ? Is length the correct measure? Consider animal welfare and accuracy /reproducibility/practicality of method

iv. How should the behavioural impact of a lesion be measured and the recovery process monitored? What are the factors that may confound long term (several months) monitoring of recovery in cephalopods? 

19:30 Dinner


Thursday , 26th September


Session 4: Ongoing research into regeneration in cephalopods

09:00 - 09:20 A. Di Cosmo (Italy) - Neurogenesis in the central nervous system of Octopus vulgaris

09:20 - 09:40 N. Tublitz (USA) - Processes and mechanisms underlying fin and arm regeneration in the European cuttlefish Sepia officinalis

09:40 - 10:00 T. Shaw (UK) - The acute wound response in Octopus vulgaris - commonalities and differences with mammalian observations

10:00 - 10:20 L. Zullo (Italy) - Identification of acetylcholinesterase in Octopus vulgaris: Expression pattern during arm regeneration  

10:20 - 10:40 G. Petrosino (Italy) - The transcriptome as a tool for discovery the molecular secrets of Octopus vulgaris

10:40 - 11:00 P. Andrews (UK) - The potential impact of Directive 2010/63/EU on regenerations studies in cephalopods
11:00 - 11:20 Coffee break

11:20 - 13:20 Session 5: Round table discussion

i. What are the ethical and welfare issues involved in regeneration and wound healing studies in cephalopods assuming that a scientific justification can be made for the studies?

ii. Can we agree on a common key features of protocols for performing studies (e.g. lesion, anaesthesia/analgesia, humane end points and health monitoring)?

iii. What are the key questions (behavioural, ecological, physiological and molecular) that can be studied in cephalopods that are not possible/difficult to study in other invertebrates and in vertebrates? Can studies be justified other than by biomedical relevance?

13:20 -14:20 Lunch

14:30 - 16:30 Session 6: Next steps

i. Are studies of cephalopods of biomedical relevance (a question for both grants and ethical review)? What information is needed for cephalopods to become established as "biomedical models for regeneration"? Are studies of one species more relevant than another?

ii. What are the factors limiting research in this area (e.g. knowledge of cephalopod genome)?

iii. Does this field have critical mass?

iv. Opportunities for collaboration and funding

v. Publication of a review from the meeting (target journal/leads)



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Thursday the 16th. 2015